»Mary in the Gospel of John« is a theme of the thesis, which is dedicated to the role and epistles of the Blessed Virgin Mary in John 2: 1-12 and John 19: 25-27. The first part of the paper presents a brief account of Mary's character and work in the Synoptic Gospels and in the Gospel of John, which aims to point out the similarities and differences, and the peculiarities of each individual evangelist, given the character of the Blessed Virgin Mary. The second, central part, is devoted to a detailed exegetical-theological analysis of the account of the mother of Jesus in the Gospel of John. It is a report of a wedding in Cana (Jn 2: 1-12) in which John portrays Mary as mediator and a report of the Passion of Jesus (Jn 19: 25-27) in which John portrays Mary as mother. The Gospel of John, which is in many ways different from the synoptic ones, brings with it the special character of Mary's character in her theology and approach. The report of the wedding in Cana (Jn 2: 1-12) is made only by John; it remains only for him to see the mother of the Lord as one who notices the needs of men and mediates for them with his Son. In the beginning of Jesus' public activity, Mary has a role to play as in the events in Cana, and she follows her course of faith and action all the way to Golgotha, under the cross of Jesus (Jn 19: 25-27). Mary is quietly present in Jesus' earthly life, but also in the environment of Jesus' disciples, the future Church. Here under the cross, as the last manifestation of the Father's will, Jesus establishes a mother-son relationship between Mary and the beloved disciple who has just introduced the future community of Jesus' disciples.